Laboratório de Biotecnologia Vegetal
MED-UALG
Desenvolvemos investigação sobre o uso de abordagens biotecnológicas para a conservação e o uso sustentável de recursos genéticos vegetais e sobre a caracterização biológica e química de compostos naturais de origem vegetal.
Equipa
Responsável
Anabela Maria Lopes Romano
Investigador
María José Aliaño González
Investigador
José M. P. T. Leitão
Investigador
Hugo Manuel Matias Duarte
Investigador
Bruno Filipe Figueiras Medronho
Investigador
Sandra Marisa Gomes Gonçalves
Investigador
Alfredo Jaime Morais Cravador
Investigador
Raquel Rodríguez Solana
Investigador
Natacha Rodrigues Coelho
Investigador - Estudante de Doutoramento
Inês Filipa Paixão Mansinhos
Contactos
Campus de Gambelas, Ed. 8, lab. 3.43 8005-139 Faro, Portugal
*
Gabinete: +351 289 800 910 Lab: +351 289 800 900 Ext.: 7323* Custo de chamada rede fixa nacional
Investigação
A investigação a decorrer no Laboratório de Biotecnologia Vegetal da Universidade do Algarve enquadra-se nas seguintes áreas principais:
Cultura de tecidos vegetais: nesta linha de investigação, utilizamos as técnicas de cultura de tecidos vegetais para a propagação de espécies de plantas silvestres em risco de extinção, aromáticas/medicinais e plantas com interesse agronómico. Até ao momento foram desenvolvidos protocolos de propagação in vitro para diversas espécies, tais como Ceratonia siliqua, Drosera intermedia, Drosophyllum lusitanicum, Lavandula viridis, Pinguicula lusitanica, Thymus lotocephalus, Tuberaria globularifolia var. major, entre outras. Estas metodologias permitem a propagação de plantas uniformes em ambiente controlado independentemente das variações sazonais, e, por isso, têm sido uma ferramenta útil para outros estudos, nomeadamente: i) para estudar a resposta fisiológica e (bio)química de espécies da família Lamiaceae a condições de stress abiótico (seca, temperatura, salinidade e radiação UVB) com especial enfase na produção de compostos fenólicos e óleos essenciais; e ii) para avaliar a eficácia da manipulação da composição do meio de cultura e da eliciação no aumento da produção de compostos fenólicos com interesse industrial, tendo em vista a sua produção sustentável.
Conservação de germoplasma: em todo o mundo, muitas espécies de plantas estão ameaçadas de extinção, especialmente devido às várias atividades humanas. No entanto, as plantas fazem parte do nosso património natural e é nossa responsabilidade preservá-las e protegê-las para as gerações futuras. A aplicação de técnicas de conservação baseadas na biotecnologia tem dado uma grande contribuição para a conservação de plantas. O objetivo não é substituir os métodos tradicionais de conservação, mas sim complementar e melhorar os métodos já existentes. A biotecnologia pode fornecer ferramentas não apenas para a conservação do germoplasma, mas também para a sua gestão e utilização. O campo de aplicação vai desde a conservação de espécies silvestres ameaçadas até ao armazenamento de genótipos ou material geneticamente modificado de espécies economicamente importantes. No Laboratório de Biotecnologia Vegetal, temos contribuído para a conservação ex situ de várias espécies silvestres da região Algarvia, incluindo espécies endémicas e em risco de extinção, recorrendo a diferentes ferramentas biotecnológicas. Para além de desenvolvermos protocolos de propagação in vitro, baseados na cultura de tecidos, também realizamos ensaios de criopreservação. A criopreservação é um tipo de conservação de longa duração e pode ser aplicada a diferentes tipos de tecidos, incluindo sementes, ápices ou segmentos nodais. Sementes de Thymus lotocephalus, Tuberaria globularifolia var. major e Plantago algarbiensis, espécies endémicas e raras do Algarve, foram criopreservadas com sucesso. Desenvolvemos protocolos de criopreservação de ápices (T. lotocephalus e T. globularifolia), e segmentos nodais (P. algarbiensis), usando os métodos de criopreservação por vitrificação, vitrificação-droplet e encapsulação-desidratação. Mais recentemente, começámos a testar a viabilidade do uso de solventes eutécticos profundos de origem natural (NADES), como substituto das soluções crioprotectoras convencionais usadas nos métodos baseados na vitrificação, que são muitas vezes tóxicas para as células vegetais. Os NADES poderão ser uma alternativa verde e menos nociva para os tecidos vegetais, contribuindo para o desenvolvimento de novas estratégias de criopreservação não só para as espécies referidas anteriormente, como para outras vulneráveis. Para além da micropropagação e criopreservação, também realizamos estudos de diversidade genética de populações destas espécies, que poderão contribuir para a criação de estratégias efetivas de conservação, prevenindo o declínio das mesmas.
Caracterização química e biológica de matrizes de plantas silvestres e produtos agroalimentares: dada a procura crescente de produtos de origem natural, principalmente de origem vegetal, e a importância da valorização da flora Mediterrânica, esta área de investigação visa a otimização de processos de extração de compostos bioativos de matrizes vegetais, dando prioridade à utilização de metodologias e solventes verdes, e o posterior estudo fitoquímico e de bioatividade dos extratáveis obtidos. Neste contexto, temos priorizadoo estudo de espécies silvestres de plantas comestíveis e/ou medicinais e produtos agroalimentares tradicionais do Algarve e, à utilização de solventes eutécticosprofundos de origem natural (NADES) em alternativa aos solventes orgânicos maistóxicos. Os estudos fitoquímicos incluem essencialmente análise do perfil fenólico e/ou de voláteis. A avaliação da bioatividade inclui vários testes in vitro, com vista à avaliação do potencial medicinal ou cosmético (antioxidante, inibitório de enzimas, fotoprotetor) dos extratos/produtos obtidos.Estudos combinados do metaboloma e do transcritoma de Phlomis purpurea a fim de elucidar os mecanismos na base da auto imunidade desta planta ao agente patogénico altamente destrutivo Phytophthora cinnamomi.Para além da determinação do envolvimento dos metabolitos secundários na defesa de P. purpurea é de esperar que novas substâncias com potencial terapêutico sejam descobertas, dado que uma grande quantidade de metabolitos secundários com actividade farmacológica tem sido isolada do género Phlomis.Estudo do potencial de P. purpurea como antagonista no controlo biológico de agentes fitopatogénicos, visando implementar estratégias ecologicamente mais seguras de que o uso de fungicidas ou outras moléculas nocivas, para prevenir a destruição das raízes em plantações e espécies florestais.Estudo experimentais em sobreirais para avaliar a capacidade de P. purpurea de erradicar P. cinnamomi.
Manipulação e valorização de recursos renováveis: a extração e dissolução de compostos de valor acrescentado (como polifenóis) e biopolímeros naturais (como celulose, lignina, quitina, goma de alfarroba e fibroina) é muito relevante num contexto de valorização de resíduos agroflorestais. O nosso trabalho tem incidido fundamentalmente no desenvolvimento de novos solventes, como sistemas eutécticos binários e ternários. Têm sido explorados preferencialmente sistemas constituídos por catiões e aniões de origem biológica, como os obtidos de ácidos orgânicos naturais, aminoácidos, adoçantes não nutritivos ou compostos naturais, como colina ou betaína. Esta linha de investigação procura ainda perceber os aspetos fundamentais (interações intermoleculares) que determinam ou condicionam a performance de extração e/ou dissolução de determinado solvente. A regeneração (precipitação, gelificação) dos biopolimeros, mecanismos envolvidos e desenvolvimento de novos materiais, como compósitos, filmes, espumas, resinas e partículas é outra vertente da linha de investigação. Neste contexto, temos procurado desenvolver filmes para aplicações quer no embalamento quer enquanto materiais triboelétricos. Por outro lado, as espumas desenvolvidas têm sido exploradas enquanto matrizes para remoção de agentes poluentes de meios aquosos. São ambicionadas também as novas funcionalidades dos biopolimeros, com o intuito de aumentar a sua versatilidade e aplicabilidade. Novos derivados de celulose e lignina têm sido desenvolvidos (alterando a carga e grau de hidrofobicidade) com o objetivo da sua incorporação em formulações cosméticas (condicionadores para cabelo) ou enquanto agentes floculantes de microplásticos ou até para desenvolvimento de novos hidrogéis para aplicações agrícolas com capacidade superior de absorção e libertação controlada de água, agentes fertilizantes e fitoquímicos. O laboratório possui ainda equipamento de caracterização reológica mecânica e ótica de vários sistemas com propriedades muito distintas (de fluidos viscosos a sólidos elásticos).
Publicações
2024
Horta Jung, M.; Maia, C.; Mora-Sala, B.; Abad-Campos, P.; Schena, L.; Mosca, S.; Carella, G.; Moricca, S.; Nechwatal, J.; Dionísio, L.; Cravador, A.; Jung, T. High diversity of Phytophthora species in natural ecosystems and nurseries of Portugal: detrimental side effect of plant introductions from the age of discovery to modern globalization. Plant Pathology, 00, 1–33. Available from: https://doi.org/10.1111/ppa.14022
T. Jung, I. Milenković, Y. Balci, J. Janoušek, T. Kudláček, Z.Á. Nagy, B. Baharuddin, J. Bakonyi, K.D. Broders, S.O. Cacciola, T.-T. Chang, N.M. Chi, T. Corcobado, A. Cravador et al. Worldwide forest surveys reveal forty-three new species in Phytophthora major Clade 2 with fundamental implications for the evolution and biogeography of the genus and global plant biosecurity. Studies in Mycology 107: 251–388 (2024). doi: 10.3114/sim.2024.107.04
Kavi Kishor P.B., Pullaiah T., Suprasanna P., Ranga Rao A., Romano A. 2024, Eds. Biotechnological Production of Bioactive Phytochemicals of Medicinal Value - A Comprehensive Treatise. Elsevier, 655 pp. ISBN: 9780443218187
P.B. Kavi Kishor, T. Pullaiah, A. Ranga Rao, A. Romano, P. Rathnagiri, and P. Suprasanna. 2024. Plants to pharmacy: Recapitulation of natural compounds transmuting human health. In: In vitro Production of bioactive phytochemicals of medicinal value: A comprehensive treatise, Kavi Kishor P.B., Pullaiah T., Suprasanna P., Ranga Rao A., Romano A. (Eds.). Elsevier. pp 1-16. ISBN: 9780443218187
Issaoui M., Oueslati S., Flamini G., Delgado A.M. & Romano A. 2024. Fortification of wheat white bread: assessing the suitability of Beta vulgaris through technological, nutritional, and sensory evaluation. International Journal of Food Science and Technology, doi:10.1111/ijfs.17396
Martins-Loucão M.A., Correia P.J. & Romano A. 2024. Carob: A Mediterranean Resource for the Future. Plants, 13, 1188, https://doi.org/10.3390/plants13091188
Djaoudene O., Bachir-bey M., Schisano C., Sabrina Djebari, Tenore G.C., Romano A. 2024. A sustainable extraction approach of phytochemicals from date (Phoenix dactylifera L.) fruit cultivars using ultrasound-assisted deep eutectic solvent: a comprehensive study on bioactivity and phenolic variability. Antioxidants, 13(2), 181, https://doi.org/10.3390/antiox13020181
Dahlström, C., Duan, R., Eivazi, A., Magalhães, S., Alves, L., Engholm, M., Svanedal, I., Edlund, H., Medronho, B., & Norgren, M. (2024). Stacking self-gluing cellulose II films: A facile strategy for the formation of novel all-cellulose laminates. Carbohydrate Polymers, 344, 122523. https://doi.org/10.1016/j.carbpol.2024.122523
Magalhães, S., Aliaño-González, M. J., Rodrigues, M., Fernandes, C., Mendes, C. V. T., Carvalho, M. G. V. S., Alves, L., Medronho, B., & Rasteiro, M. da G. (2024). Enhancing Cellulose and Lignin Fractionation from Acacia Wood: Optimized Parameters Using a Deep Eutectic Solvent System and Solvent Recovery. Molecules, 29(15), 3495. https://doi.org/10.3390/molecules29153495
Duarte, H., Aliaño-González, M. J., Romano, A., & Medronho, B. (2024). Advancements in Detection and Mitigation Strategies for Petroleum-Derived Contaminants in Aquatic Environments: A Comprehensive Review. Sensors, 24(11), 3284. https://doi.org/10.3390/s24113284
Dahlström, C., Eivazi, A., Nejström, M., Zhang, R., Pettersson, T., Iftikhar, H., Rojas, O. J., Medronho, B., & Norgren, M. (2024). Regenerated cellulose properties tailored for optimized triboelectric output and the effect of counter-tribolayers. Cellulose, 31(4), 2047–2061. https://doi.org/10.1007/s10570-024-05745-8
Gericke, M., Amaral, A. J. R., Budtova, T., De Wever, P., Groth, T., Heinze, T., Höfte, H., Huber, A., Ikkala, O., Kapuśniak, J., Kargl, R., Mano, J. F., Másson, M., Matricardi, P., Medronho, B., Norgren, M., Nypelö, T., Nyström, L., Roig, A., … Fardim, P. (2024). The European Polysaccharide Network of Excellence (EPNOE) research roadmap 2040: Advanced strategies for exploiting the vast potential of polysaccharides as renewable bioresources. Carbohydrate Polymers, 326, 121633. https://doi.org/10.1016/j.carbpol.2023.121633
Ribau Teixeira, M., Ismail, A., Medronho, B., Alves, L., Pedrosa, J. F. S., Ferreira, P. J. T., Serrão Sousa, V., & Rosa da Costa, A. M. (2024). Nanofibrillated cationic cellulose derivatives as flocculants for domestic wastewater treatment. Journal of Water Process Engineering, 58, 104817. https://doi.org/10.1016/j.jwpe.2024.104817
Melro, E., Duarte, H., Eivazi, A., Costa, C., Faleiro, M. L., da Costa, A. M. R., Antunes, F. E., Valente, A. J. M., Romano, A., Norgren, M., & Medronho, B. (2024). Poly(butylene succinate)-Based Composites with Technical and Extracted Lignins from Wood Residues. ACS Applied Polymer Materials, 6(2), 1169–1181. https://doi.org/10.1021/acsapm.3c02103
Duarte, H., Aliaño-González, M. J., Cantos-Villar, E., Faleiro, L., Romano, A., & Medronho, B. (2024). Sustainable extraction of polyphenols from vine shoots using deep eutectic solvents: Influence of the solvent, Vitis sp., and extraction technique. Talanta, 267, 125135. https://doi.org/10.1016/j.talanta.2023.125135
Duarte, T. A. G., Pereira, R. F. P., Medronho, B., Maltseva, E. S., Krivoshapkina, E. F., Varela-Dopico, A., Taboada, P., Fu, L., Ferreira, R. A. S., & de Zea Bermudez, V. (2024). A Glance at Novel Ionanofluids Incorporating Silk-Derived Carbon Dots. Chemistry of Materials, 36(3), 1136–1152. https://doi.org/10.1021/acs.chemmater.3c01370
Mansinhos I., Gonçalves S., Rodríguez-Solana R., Pereira-Caro G., Moreno-Rojas J., Romano A., 2024. Nutrient deficiency-induced stress improves skincare effects and phytochemical content of green extracts from Lamiaceae in vitro cultures. Horticulturae 10, 947. https://doi.org/10.3390/horticulturae10090947.
Hamdoun O., Gonçalves S., Mansinhos I., Rodríguez-Solana R., Pereira-Caro G., Moreno-Rojas, J.M., El Kbiach M., El Bouzdoudi B., Romano A., 2024. Phytochemical characterization and bioactivity of extracts from different fruit parts of Opuntia leucotricha DC.: A comparison between a conventional organic solvent and green natural deep eutectic solvents. Horticulturae, 10, 824. https://doi.org/10.3390/horticulturae10080824.
Mansinhos I., Gonçalves S., Rodríguez-Solana R., Moreno-Rojas J.M., Romano A., 2024. Environmental factors related to climate change alter the chemical composition and biological activity of Lavandula viridis L’Hér essential oil. Agriculture, 14, 1067. https://doi.org/10.3390/agriculture14071067.
Mansinhos I., Gonçalves S., Romano A., 2024. How climate change-related abiotic factors affect the production of industrial valuable compounds in Lamiaceae plant species: a review. Frontiers in Plant Science, 15, 1370810. https://doi.org/10.3389/fpls.2024.1370810.
Das G., Ortega L.A.J., Gonçalves S., Basilio Heredia J., Gomes Pereira M.L., Romano A., Shin H.-S., Patra J.K., 2024. Anti-obesogenic effects of plant natural products: A focus on Korean traditional foods. Trends in Food Science and Technology, 148, 104470. https://doi.org/10.1016/j.tifs.2024.104470.
Gonçalves S., Romano A., 2024. In vitro production of secondary metabolites by Thymus plants. In Biotechnological Production of Bioactive Phytochemicals of Medicinal Value. A Comprehensive Treatise, Kavi Kishor P.B., Pullaiah T., Suprasanna P., Rao P., Romano A. (Eds.), Elsevier. Chapter 4 (pp. 103-123). Paperback ISBN: 9780443218187; eBook ISBN: 9780443218194. https://doi.org/10.1016/B978-0-443-21818-7.00001-0.
Mansinhos I., Gonçalves S., Romano A., 2024. Impact of abiotic stresses on in vitro production of secondary metabolites. In: In Vitro Propagation and Secondary Metabolite Production from Medicinal Plants: Current Trends (Part 1), Anis M., Khanam M.N. (Eds.), Bentham Science Publishers. Chapter 4 (pp. 62–90). https://doi.org/10.2174/9789815165227124010007.
Gonçalves S., Mansinhos I., Romano A., 2024. Influence of biotic and abiotic factors on the production and composition of essential oils. In Essential Oils: Extraction Methods and Applications, Inamuddin M., Altalhi, T., Neves Cruz J., (Eds.), Wiley Online Library. Chapter 4 (pp. 69–97). ISBN: 978-111982961-4, 978-111982935-5. https://doi.org/10.1002/9781119829614.ch4.
2023
Neves, D.; Figueiredo, A.; Maia, M.; Laczko, E.; Pais, M.S.; Cravador, A. A Metabolome Analysis and the Immunity of Phlomis purpurea against Phytophthora cinnamomi. Plants, 12, 1929 (2023). https://doi.org/10.3390/plants12101929
Djaoudene O., Romano A., Bradai Y.D., Zebiri F., Ouchene A., Yousfi Y., Amrane-Abider M., Sahraoui-Remini Y., Madani K. 2023. A Global Overview of Dietary Supplements: Regulation, Market Trends, Usage during the COVID-19 Pandemic, and Health Effects. Nutrients 15, 3320. https://doi.org/10.3390/nu15153320. (This paper is featured by the USA's National Institute of Health, Office of Dietary Supplements)
Delgado A., Gonçalves S., Romano A. 2023. Mediterranean Diet: the role of phenolic compounds from aromatic plant foods. Foods, 12, 840. https://doi.org/10.3390/foods12040840
Romano A., Cruz A.L., Gonçalves A., Quintas C., Esteves E., Bernardes J., Mateus M.P., Braz N., Coord. A. Freitas. 2023. Plano de Atividades para a salvaguarda da Dieta Mediterrânica na região do Algarve (2023-2027). ISBN: 978-989-9127-62-3, DOI: https://doi.org/10.34623/b5m4-w049
Duarte, H., Carrera, C., Aliaño-González, M. J., Gutiérrez-Escobar, R., Jiménez-Hierro, M. J., Palma, M., Galego, L., Romano, A., & Medronho, B. (2023). On the Valorization of Arbutus unedo L. Pomace: Polyphenol Extraction and Development of Novel Functional Cookies. Foods, 12(19), 3707. https://doi.org/10.3390/foods12193707
Duarte, H., Gomes, V., Aliaño-González, M. J., Faleiro, L., Romano, A., & Medronho, B. (2023). Optimization of the extraction of polyphenols from Pinus pinaster residues using deep eutectic solvents: a sustainable approach. Wood Science and Technology, 57(5), 1175–1196. https://doi.org/10.1007/s00226-023-01493-3
Melro, E., Duarte, H., Antunes, F. E., Valente, A. J. M., Romano, A., Norgren, M., & Medronho, B. (2023). Engineering novel phenolic foams with lignin extracted from pine wood residues via a new levulinic-acid assisted process. International Journal of Biological Macromolecules, 248, 125947. https://doi.org/10.1016/j.ijbiomac.2023.125947
Magalhães, S., Fernandes, C., Pedrosa, J. F. S., Alves, L., Medronho, B., Ferreira, P. J. T., & Rasteiro, M. da G. (2023). Eco-Friendly Methods for Extraction and Modification of Cellulose: An Overview. Polymers, 15(14), 3138. https://doi.org/10.3390/polym15143138
Melro, E., Riddell, A., Bernin, D., da Costa, A. M. R., Valente, A. J. M., Antunes, F. E., Romano, A., Norgren, M., & Medronho, B. (2023). Levulinic Acid-Based “Green” Solvents for Lignocellulose Fractionation: On the Superior Extraction Yield and Selectivity toward Lignin. Biomacromolecules, 24(7), 3094–3104. https://doi.org/10.1021/acs.biomac.3c00169
Norgren, M., Costa, C., Alves, L., Eivazi, A., Dahlström, C., Svanedal, I., Edlund, H., & Medronho, B. (2023). Perspectives on the Lindman Hypothesis and Cellulose Interactions. Molecules, 28(10), 4216. https://doi.org/10.3390/molecules28104216
Fernandes, C., Medronho, B., Alves, L., & Rasteiro, M. G. (2023). On Hair Care Physicochemistry: From Structure and Degradation to Novel Biobased Conditioning Agents. Polymers, 15(3). https://doi.org/10.3390/polym15030608
Delgado A., Gonçalves S., Romano A., 2023. Mediterranean Diet: the role of phenolic compounds from aromatic plant foods. Foods, 12, 840. https://doi.org/10.3390/foods12040840
Medronho B., Pereira A., Duarte H., Gentile L., Costa A., Romano A., Olsson U., 2023. Probing cellulose–solvent interactions with self-diffusion NMR: Onium hydroxide concentration and co-solvent effects. Carbohydrate Polymers, 303, 120440. https://doi.org/10.1016/j.carbpol.2022.120440
Erazo Solorzano, C. Y., Disca, V., Muñoz-Redondo, J. M., Tuárez García, D. A., Sánchez-Parra, M., Carrilo Zenteno, M. D., Moreno-Rojas, J. M., Rodríguez-Solana, R., 2023. Effect of drying technique on the volatile content of Ecuadorian Bulk and Fine-Flavor cocoa. Foods, 12(5), 1065. https://doi.org/10.3390/foods12051065
Muñoz-Redondo, J. M., Puertas, B., Valcárcel-Muñoz, M. J., Rodríguez-Solana, R., Moreno-Rojas, J.M., 2023. Impact of stabilization method (traditional versus cold) and filtration step on the ester profile of “Brandy de Jerez”. A chemometric assessment. Applied Science. Accepted. https://doi.org/10.3390/app13063428
Gutiérrez-Escobar, R.; Aliaño-González, M.J.; Marrufo-Curtido, A.; Jiménez-Hierro, M.J.; Puertas, B.; Cantos-Villar, E., 2023. Sulfur Dioxide-Free Verdejo Wines through the Use of a Pure Stilbene Extract: Exploring Possible Synergistic Effect with Glutathione. Journal of the Science of Food and Agriculture, 103, 1152–1160. https://doi.org/10.1002/jsfa.12209
2022
Maia, C.; Horta Jung, M.; Carella, G.; Milenković, I.; Janoušek, J.; Tomšovský, M.; Mosca, S.; Schena, L.; Cravador, A.; Moricca, S.; Jung, T. Eight new Halophytophthora species from marine and brackish-water ecosystems in Portugal and an updated phylogeny for the genus. Persoonia 48: 54–90 (2022). https://doi.org/10.3767/persoonia.2022.48.02
Mansinhos I., Gonçalves S., Rodríguez Solana R., Ordóñez-Díaz J.L., Moreno-Rojas J.M., Romano A., 2022. Impact of Temperature on Phenolic and Osmolyte Contents in In Vitro Cultures and Micropropagated Plants of Two Mediterranean Plant Species, Lavandula viridis and Thymus lotocephalus. Plants, 11, 3516. https://doi.org/10.3390/plants11243516
Duarte H., Gomes V., Aliaño-González M.J., Medronho B., Faleiro F. & Romano A., 2022. Ultrasound–Assisted Extraction of Polyphenols from Maritime Pine Residues with Deep Eutectic Solvents. Foods, 11(23), 3754. https://doi.org/10.3390/foods11233754
Duarte H., Gummel J., Robles E., Berti D. & Fratini E., 2022. Ultra-/Small Angle X-ray Scattering (USAXS/SAXS) and Static Light Scattering (SLS) Modeling as a Tool to Determine Structural Changes and Effect on Growth in S. epidermidis. ACS Appl. Bio Mater. https://doi.org/10.1021/acsabm.2c00218
Barea-Sepúlveda M., Duarte H., Aliaño-González M.J., Romano A. & Medronho B., 2022. Total Ion Chromatogram and Total Ion Mass Spectrum as Alternative Tools for Detection and Discrimination (A Review). Chemosensors 2022, 10, 465. (Selected by the editors as the issue cover). https://doi.org/10.3390/chemosensors10110465
Mansinhos I., Gonçalves S., Rodríguez Solana R., Duarte H., Ordóñez-Díaz J.L., Moreno-Rojas J.M., Romano A., 2022. Response of Thymus lotocephalus In Vitro Cultures to Drought Stress and Role of Green Extracts in Cosmetics. Antioxidants 11, 1475. https://doi.org/10.3390/antiox11081475
Baca-Bocanegra B., Gonçalves S., Nogales-Bueno J., Mansinhos I., Heredia F.J., Hernández-Hierro J.M., Romano A., 2022. Influence of wine pH and ethanol content on the fining efficacy of proteins from winemaking byproducts. Foods 11, 1688. https://doi.org/10.3390/foods11121688
Magalhães S., Alves L., Romano A., Medronho B., Rasteiro, M.G., 2022. Extraction and characterization of microplastics from Portuguese industrial effluents. Polymers, 14, 2902. https://doi.org/10.3390/polym14142902
Magalhães S., Moreira A., Almeida R., Cruz P., Alves L., Costa C., Mendes C., Medronho B., Romano A., Carvalho M.G., Gamelas J., Rasteiro, M.G., 2022. Acacia wood fractionation using deep eutectic solvents: extraction, recovery and characterization of the different fractions. ACS Omega, 7, 30, 26005–26014. https://doi.org/10.1021/acsomega.1c07380
Melro E., Antunes F.E., Valente A.J.M., Duarte H., Romano A., Medronho B., 2022. On the development of phenol-formaldehyde resins using a new type of lignin extracted from pine wood with a levulinic-acid based solvent. Molecules, 27, 27(9):2825. https://doi.org/10.3390/molecules27092825
Das G., Gonçalves S., Heredia J.B., Romano A., Jiménez-Ortega L.A., Gutiérrez-Grijalva E.P., Shin H.S., Patra J.K., 2022. Cardiovascular protective effect of cinnamon and its major bioactive constituents: An update. Journal of Functional Foods, pp. 105045. https://doi.org/10.1016/j.jff.2022.105045
Aliaño-González, M.J.; Gabaston, J.; Ortiz-Somovilla, V.; Cantos-Villar, E., 2022. Wood Waste from Fruit Trees: Biomolecules and Their Applications in Agri-Food Industry. Biomolecules, 12, 238. https://doi.org/10.3390/biom12020238
Muñoz-Redondo, J. M., Valcárcel-Muñoz, M. J., Solana, R. R., Puertas, B., Cantos-Villar, E., & Moreno-Rojas, J. M., 2022. Development of a methodology based on headspace solid-phase microextraction coupled to gas chromatography-mass spectrometry for the analysis of esters in brandies. Journal of Food Composition and Analysis, 108, 104458. https://doi.org/10.1016/j.jfca.2022.104458
Kaur, I., Sharma, A. D., Samtiya, M., Pereira-Caro, G., Rodríguez-Solana, R., Dhewa, T., & Moreno-Rojas, J. M., 2022. Potential of bioactive compounds derived from underutilized fruit-bearing plants: a comprehensive review. European Food Research and Technology, 1-20. https://doi.org/10.1007/s00217-022-04171-z
Sánchez-Parra, M., Lopez, A., Muñoz-Redondo, J. M., Montenegro-Gómez, J. C., Pérez-Aparicio, J., Pereira-Caro, G., Rodríguez-Solana, R., Moreno-Rojas, J. M. & Ordóñez-Díaz, J. L., 2022. Study of the influence of the fishing season and the storage temperature in the fishing vessel on the biogenic amine and volatile profiles in fresh yellowfin tuna (Thunnus albacares) and dry-cured mojama. Journal of Food Composition and Analysis, 114, 104845. https://doi.org/10.1016/j.jfca.2022.104845
Barea-Sepúlveda, M.; Duarte, H.; Aliaño-González, M.J.; Romano, A.; Medronho, B., 2022. Total Ion Chromatogram and Total Ion Mass Spectrum as Alternative Tools for Detection and Discrimination (A Review). Chemosensors, 10, 465. https://doi.org/10.3390/chemosensors10110465
Calle, J.L.P.; Falatová, B.; Aliaño-González, M.J.; Ferreiro-González, M.; Palma, M., 2022. Machine Learning Approaches over Ion Mobility Spectra for the Discrimination of Ignitable Liquids Residues from Interfering Substrates. Talanta Open, 6, 100125. https://doi.org/10.1016/j.talo.(2022).100125
Alavarsa-Cascales, D.; Aliaño-González, M.J.; Palma, M.; Barbero, G.F.; Carrera, C., 2022. Optimization of an Enzyme-Assisted Extraction Method for the Anthocyanins Present in Açai (Euterpe Oleracea Mart.). Agronomy, 12, 2327. https://doi.org/10.3390/agronomy12102327
Aliaño-González, M.J.; Barea-Sepúlveda, M.; Espada-Bellido, E.; Ferreiro-González, M.; López-Castillo, J.G.; Palma, M.; Barbero, G.F.; Carrera, C., 2022. Ultrasound-Assisted Extraction of Total Phenolic Compounds and Antioxidant Activity in Mushrooms. Agronomy, 12, 1812. https://doi.org/10.3390/agronomy12081812
Aliaño-González, M.J.; Montalvo, G.; García-Ruiz, C.; Ferreiro-González, M.; Palma, M., 2022. Assessment of Volatile Compound Transference through Firefighter Turnout Gear. International Journal of Environmental Research and Public Health, 19, 3663. https://doi.org/10.3390/ijerph19063663
Duarte, H.; Gomes, V.; Aliaño-González, M.J.; Faleiro, L.; Romano, A.; Medronho, B., 2022. Ultrasound-Assisted Extraction of Polyphenols from Maritime Pine Residues with Deep Eutectic Solvents. Foods, 11, 3754. https://doi.org/10.3390/foods11233754
Aliaño-González, M.J.; Carrera, C.; Barbero, G.F.; Palma, M., 2022. A Comparison Study between Ultrasound–Assisted and Enzyme–Assisted Extraction of Anthocyanins from Blackcurrant (Ribes Nigrum L.). Food Chemistry: X, 13, 100192. https://doi.org/10.1016/j.fochx.2021.100192
Piñeiro, Z.; Aliaño-González, M.J.; González-de-Peredo, A.V.; Palma, M.; de Andrés, M.T., 2022. Microwave-Assisted Extraction of Non-Coloured Phenolic Compounds from Grape Cultivars. European Food Research Technology, 248, 1883–1901. https://doi.org/10.1007/s00217-022-04013-y
Vázquez-Espinosa, M.; González-de-Peredo, A.V.; Carrera, C.; Palma, M.; Barbero, G.F.; Aliaño-González, M.J., 2022. Ultrasound-Assisted Extraction of Betalains from Opuntia Fruit Pulp of Different Color Varieties. Agronomy, 12, 2604. https://doi.org/10.3390/agronomy12112604
Maia, C., Horta Jung, M., Carella, G., .Moricca, S., Jung, T., 2022. Eight new Halophytophthora species from marine and brackish-water ecosystems in Portugal and an updated phylogeny for the genus. Persoonia: Molecular Phylogeny and Evolution of Fungi, 48, 54–90. https://doi.org/10.3767/persoonia.2022.48.02
Reis, J.M., Pereira, R.J., Coelho, P.S., Leitão, J.M., 2022. Assessment of Wild Rocket (Diplotaxis tenuifolia (L.) DC.) Germplasm Accessions by NGS Identified SSR and SNP Markers. Plants, 11(24), 3482. https://doi.org/10.3390/plants11243482
Coelho, P.S., Reis, J.M., Pereira, A.L., Lopes, V., Leitão, J.M., 2022. Downy mildew resistance and genetic variability in a wild rocket germplasm collection. Agronomy Journal, 114(6), 3083–3095. https://doi.org/10.1002/agj2.21190
2021
Baldé, Aladje; Pais, Maria Salomé S.; Cravador Alfredo. Innate Immunity of Phlomis purpurea against Phytophthora cinnamomi: A Transcriptomic Analysis. Journal of Agricultural Science and Technology A, 11 22-46 (2021). doi: 10.17265/2161-6256/2021.01.002
Coelho N., Romano A., 2021. Impact of chitosan on plant tissue culture: recent applications. Plant Cell, Tissue and Organ Culture 148: 1–13. https://doi.org/10.1007/s11240-021-02156-6
Costa, Carolina; Medronho, Bruno; Eivazi, Alireza; Svanedal, Ida; Lindman, Björn; Edlund, Håkan; Norgren, Magnus., 2021. Lignin enhances cellulose dissolution in cold alkali. Carbohydrate Polymers, 274, 118661. https://doi.org/10.1016/j.carbpol.2021.118661
Alireza Eivazi; Bruno Medronho; Björn Lindman; Magnus Norgren., 2021. On the Development of All-Cellulose Capsules by Vesicle-Templated Layer-by-Layer Assembly. Polymers, 13, 589-589. https://doi.org/10.3390/polym13040589
Björn Lindman; Bruno Medronho; Luís Alves; Magnus Norgren; Lars Nordenskiöld., 2021. Hydrophobic interactions control the self-assembly of DNA and cellulose. Quarterly Reviews of Biophysics, 54. https://doi.org/10.1017/S0033583521000019
Costa C., Medronho B., Filipe A., Romano A., Lindman B., Edlund H., Norgren M., 2021. On the formation and stability of cellulose-based emulsions in alkaline systems: effect of the solvent quality. Carbohydrate Polymers. https://doi.org/10.1016/j.carbpol.2022.119257
Pan Y.-L., Rodrigues M.J., Pereira C.G., Engrola S., Colen R., Mansinhos I., Romano A., Andrade P., Fernandes F., Custódio L., 2021. Exploring the biotechnological value of marine invertebrates: a closer look at the biochemical and antioxidant properties of Sabella spallanzanii and Microcosmus squamiger. Animals, 11(12): 3557. https://doi.org/10.3390/ani11123557
Gonçalves S., Mansinhos I., Rodríguez Solana R., Pereira-Caro G., Moreno-Rojas J.M., Romano A., 2021. Impact of metallic nanoparticles on in vitro culture, phenolic profile and biological activity of two Mediterranean Lamiaceae species: Lavandula viridis L’Hér and Thymus lotocephalus G. López and R. Morales. Molecules, 26, 6427. https://doi.org/10.3390/molecules26216427
Rukavina I, Rodrigues M.J., Pereira C.G., Mansinhos I., Romano A., Ślusarczyk S., Matkowski A., Custódio L., 2021. Greener is better: first approach to use natural deep eutectic solvents (NADES) to extract antioxidants from the medicinal halophyte Polygonum maritimum L. Molecules, 26: 6136. https://doi.org/10.3390/molecules26206136
Mansinhos I., Gonçalves S., Rodríguez Solana R., Ordóñez-Díaz J.L., Moreno-Rojas J.M., Romano A., 2021. Ultrasonic-Assisted Extraction (UAE) and NADES combination: A green strategy to improve the recovery of phenolic compounds from Lavandula pedunculata subsp. lusitanica (Chaytor) Franco. Antioxidants, 10, 582. https://doi.org/10.3390/antiox10040582
Rodríguez-Solana R., Romano A., Moreno-Rojas J.M., 2021. Carob Pulp: A Nutritional and Functional By-Product Worldwide Spread in the Formulation of Different Food Products and Beverages. A Review. Processes, 9, 1146. https://doi.org/10.3390/pr9071146
Coelho N., Filipe A., Medronho B., Magalhães S., Vitorino C., Alves L., Gonçalves S., Romano A., 2021. Rheological and microstructural features of plant culture media doped with biopolymers: influence on the growth and physiological responses of in vitro grown shoots of Thymus lotocephalus. Polysaccharides, 2, 538–553. https://doi.org/10.3390/polysaccharides2020032
Das G., Shin H.-S., Tundis R., Gonçalves S., Ourlad Alzeus G. Tantengco, Acquaviva, Campos M., Acquaviva R., Malfa G.A., Romano A., Robles J.A.H., Clores M.Q., Patra J.K., 2021. Plant species of sub-family Valerianaceae: a review on its effect on the central nervous system. Plants, 10, 846, Review paper. https://doi.org/10.3390/plants10050846
Melro E., Filipe A., Sousa D., Medronho B. & Romano A., 2021. Revisiting lignin: a tour through its structural features, characterization methods and applications. New Journal of Chemistry, 45: 6986 - 7013 (Cover). https://doi.org/10.1039/D0NJ06234K
Melro E., Valente A.J.M, Antunes F.E., Romano A., Medronho B., 2021. Enhancing lignin dissolution and extraction: The effect of surfactants. Polymers, 13, 714. https://doi.org/10.3390/polym13050714
Magalhães S., Filipe A., Fernandes C., Melro E., Vitorino C., Alves L., Romano A., Rasteiro M.A. & Medronho B., 2021. Lignin extraction from waste pine sawdust using biomass derived solvent system. Polymers, 13(7), 1090. https://doi.org/10.3390/polym13071090
Fernandes C., Melro E., Magalhães S., Alves L., Craveiro R., Filipe A., Valente A.J.M., Martins G., Antunes F.E., Romano A., Medronho B., 2021. New deep eutectic solvent assisted extraction of highly pure lignin from maritime pine sawdust (Pinus pinaster). International Journal of Biological Macromolecules, 177: 294-305. https://doi.org/10.1016/j.ijbiomac.2021.02.088
Djaoudenea O., Mansinhos I., Gonçalves S., Jara-Palacios M.J., Bachir M.b., Romano A.,2021. Phenolic profile, antioxidant activity and enzyme inhibitory capacities of fruit and seed extracts from different Algerian cultivars of date (Phoenix dactylifera L.) were affected by in vitro simulated gastrointestinal digestion. South African Journal of Botany, 137: 133-148. https://doi.org/10.1016/j.sajb.2020.10.015
Costa C., Rosa P., Filipe A., Medronho B., Romano A., Liberman L., Talmon Y., Norgren M., 2021. Cellulose-stabilized oil-in-water emulsions: Structural features, microrheology, and stability. Carbohydrate Polymers, 252: 117092. https://doi.org/10.1016/j.carbpol.2020.117092
Alves L., Medronho B., Filipe A., Romano A., Rasteiro M.G., Lindman B., Topgaard D., Davidovich I., Talmon Y., 2021. Revisiting the dissolution of cellulose in H3PO4 (aq.) through cryo-TEM, PTssNMR and DWS. Carbohydrate Polymers, 252: 117122. https://doi.org/10.1016/j.carbpol.2020.117122
Rodríguez-Solana R., Esteves E., Mansinhos I., Gonçalves S., Pérez-Santín E., Galego L. & Romano A., 2021. Influence of elaboration process on chemical, biological and sensory characteristics of European pennyroyal liqueurs. Journal of the Science of Food and Agriculture, 101: 4076-4089. https://doi.org/10.1002/jsfa.11043
Perez Santin, E., Rodríguez Solana, R., González García, M., García Suárez, M. D. M., Blanco Díaz, G. D., Cima Cabal, M. D., Moreno Rojas, J. M. & *Lopez Sanchez, J. I., 2021. Toxicity prediction based on artificial intelligence: A multidisciplinary overview. Wiley Interdisciplinary Reviews: Computational Molecular Science, 11(5), e1516. https://doi.org/10.1002/wcms.1516
Barreiro, R., Rodríguez-Solana, R., Alonso, L., Salinero, C., López Sánchez, J. I., & *Pérez-Santín, E., 2021. Fast 1H-NMR Species Differentiation Method for Camellia Seed Oils Applied to Spanish Ornamentals Plants. Comparison with Traditional Gas Chromatography. Plants, 10(10), 1984. https://doi.org/10.3390/plants10101984
Carrera, C.; Aliaño-González, M.J.; Rodríguez-López, J.; Ferreiro-González, M.; Ojeda-Copete, F.; Barbero, G.F.; Palma, M., 2021. Optimization of an Ultrasound-Assisted Extraction Method for the Analysis of Major Anthocyanin Content in Erica Australis Flowers. Molecules, 26, 2884. https://doi.org.10.3390/molecules26102884
Carrera, C.; Aliaño-González, M.J.; Valaityte, M.; Ferreiro-González, M.; Barbero, G.F.; Palma, M., 2021. A Novel Ultrasound-Assisted Extraction Method for the Analysis of Anthocyanins in Potatoes (Solanum Tuberosum L.). Antioxidants, 10, 1375. https://doi.org10.3390/antiox10091375
Gutiérrez-Escobar, R.; Aliaño-González, M.J.; Cantos-Villar, E., 2021. Wine Polyphenol Content and Its Influence on Wine Quality and Properties: A Review. Molecules, 26, 718. https://doi.org/10.3390/molecules26030718
Jaén-González, L.; Aliaño-González, M.J.; Ferreiro-González, M.; Barbero, G.F.; Palma, M., 2021. A Novel Method Based on Headspace-Ion Mobility Spectrometry for the Detection and Discrimination of Different Petroleum Derived Products in Seawater. Sensors, 21, 2151. https://doi.org/10.3390/s21062151
Pereira, R., Leitão, J.M., 2021. A non-rogue mutant line induced by ENU mutagenesis in paramutated rogue peas (Pisum sativum l.) is still sensitive to the rogue paramutation. Genes, 12(11), 1680. https://doi.org/10.3390/genes12111680
2020
Das G., Lim K.J., Tantengco O.A.G., Carag H.M., Gonçalves S., Romano A., Das S.K., Coy-Barrera E., Shin H.-S. Gutiérrez-Grijalva E.P., Heredia J.B. & Patra J.K., 2020. Cactus: chemical, nutraceutical composition and potential bio-pharmacological properties. Phytotherapy Research, 2020: 1–36. https://doi.org/10.1002/ptr.6889
Melro E., Filipe A., Valente A.J.M.., Antunes F.E., Romano A., Norgren M., Medronho B., 2020. Levulinic acid: a novel sustainable solvent for lignin dissolution. International Journal of Biological Macromolecules, 164: 3454-3461. https://doi.org/10.1016/j.ijbiomac.2020.08.128
Jara-Palacios M.J., Gonçalves S., Heredia F.J., Hernanz D., Romano A., 2020. Extraction of antioxidants from winemaking byproducts: effect of the solvent on phenolic composition, antioxidant and anti-cholinesterase activities, and electrochemical behaviour. Antioxidants, 9: 675. https://doi.org/10.3390/antiox9080675
Mariana Lopes; Chloé Pierrepont; Carla Margarida Duarte; Alexandra Filipe; Bruno Medronho; Isabel Sousa., 2020. "Legume Beverages from Chickpea and Lupin, as New Milk Alternatives". Foods, 9, 1458. https://doi.org/10.3390/foods9101458
Malin From; Per Tomas Larsson; Bo Andreasson; Bruno Medronho; Ida Svanedal; Håkan Edlund; Magnus Norgren., 2020. "Tuning the properties of regenerated cellulose: Effects of polarity and water solubility of the coagulation medium". Carbohydrate Polymers, 236, 116068-116068. https://doi.org/10.1016/j.carbpol.2020.116068
Yang, Jiayi; Medronho, Bruno; Lindman, Björn & Norgren, Magnus., 2020. Simple One Pot Preparation of Chemical Hydrogels from Cellulose Dissolved in Cold LiOH/Urea. Polymers (Basel), 12, 373. https://doi.org/10.3390/polym12020373
Magalhães S., Alves L., Medronho B., Romano A., Rasteiro M., 2020. Bio-based solutions for removal of microplastics in ecosystems: current trends and future perspectives. Molecules, 25(17), 3954. https://doi.org/10.3390/molecules25173954
Das G., Patra J.K., Gonçalves S., Romano A., Gutiérrez-Grijalva E.P., Basilio Heredia J., Talukdar A.D., Shome S. & Shin H-S., 2020. Galangal, the multipotent super spices: A comprehensive review. Trends in Food Science & Technology, 101: 50-62. https://doi.org/10.1016/j.tifs.2020.04.032
Coelho N., Gonçalves S. & Romano A., 2020. Endemic plant species conservation: biotechnological approaches. Plants, 9, 345; Review. [Q1 – Plant Science; IF= 2.632]. https://doi.org/10.3390/plants9030345
Melro E., Filipe A., Antunes F.E., Sousa D., Romano A., Valente A.J.M. & Medronho B., 2020. Dissolution of kraft lignin in alkaline solutions. International Journal of Biological Macromolecules, 148: 688-695. https://doi.org/10.1016/j.ijbiomac.2020.01.153
Aliaño-González, M.J.; Jarillo, J.A.; Carrera, C.; Ferreiro-González, M.; Álvarez, J.Á.; Palma, M.; Ayuso, J.; Barbero, G.F.; Espada-Bellido, E., 2020. Optimization of a Novel Method Based on Ultrasound-Assisted Extraction for the Quantification of Anthocyanins and Total Phenolic Compounds in Blueberry Samples (Vaccinium Corymbosum L.). Foods, 9, 1763. https://doi.org/10.3390/foods9121763
Aliaño-González, M.J.; Espada-Bellido, E.; Ferreiro-González, M.; Carrera, C.; Palma, M.; Ayuso, J.; Álvarez, J.Á.; F. Barbero, G., 2020. Extraction of Anthocyanins and Total Phenolic Compounds from Açai (Euterpe Oleracea Mart.) Using an Experimental Design Methodology. Part 2: Ultrasound-Assisted Extraction. Agronomy, 10, 326. https://doi.org/10.3390/agronomy10030326
Aliaño-González, M.J.; Ferreiro-González, M.; Espada-Bellido, E.; Barbero, G.F.; Palma, M., 2020. Novel Method Based on Ion Mobility Spectroscopy for the Quantification of Adulterants in Honeys. Food Control, 114, 107236. https://doi.org/10.1016/j.foodcont.2020.107236
Aliaño-González, M.J.; Ferreiro-González, M.; Espada-Bellido, E.; Carrera, C.; Palma, M.; Ayuso, J.; Barbero, G.F.; Álvarez, J.Á., 2020. Extraction of Anthocyanins and Total Phenolic Compounds from Açai (Euterpe Oleracea Mart.) Using an Experimental Design Methodology. Part 3: Microwave-Assisted Extraction. Agronomy, 10, 179. https://doi.org/10.3390/agronomy10020179
Aliaño-González, M.J.; Ferreiro-González, M.; Espada-Bellido, E.; Carrera, C.; Palma, M.; Álvarez, J.A.; Ayuso, J.; F. Barbero, G., 2020. Extraction of Anthocyanins and Total Phenolic Compounds from Açai (Euterpe Oleracea Mart.) Using an Experimental Design Methodology. Part 1: Pressurized Liquid Extraction. Agronomy, 10, 183. https://doi.org/10.3390/agronomy100(2018)3
Aliaño-González, M.J.; Richard, T.; Cantos-Villar, E., 2020. Grapevine Cane Extracts: Raw Plant Material, Extraction Methods, Quantification, and Applications. Biomolecules, 10, 1195. https://doi.org/10.3390/biom10081195
Guerrero, R.F.; Aliaño-González, M.J.; Puertas, B.; Richard, T.; Cantos-Villar, E., 2020. Comparative Analysis of Stilbene Concentration in Grapevine Shoots of Thirteen Vitis during a Three-Year Study. Industrial Crops and Products, 156, 112852. https://doi.org/10.1016/j.indcrop.2020.112852
Calle, J.L.; Ferreiro-González, M.; Aliaño-González, M.J.; F. Barbero, G.; Palma, M., 2020. Characterization of Biodegraded Ignitable Liquids by Headspace–Ion Mobility Spectrometry. Sensors, 20, 6005. https://doi.org/10.3390/s20216005
Piotr Konieczka, P.; Aliaño-González, M.J.; Ferreiro-González, M.; Barbero, G.F.; Palma, M., 2020. Characterization of Arabica and Robusta Coffees by Ion Mobility Sum Spectrum. Sensors, 20, 3123. https://doi.org/10.3390/s20113123
Putri, A.R.; Aliaño-González, M.J.; Ferreiro, M.; Setyaningsih, W.; Rohman, A.; Riyanto, S.; Palma, M., 2020. Development of a Methodology Based on Headspace-Gas Chromatography-Ion Mobility Spectrometry for the Rapid Detection and Determination of Patin Fish Oil Adulterated with Palm Oil. Arabian Journal of Chemistry, 13, 7524–7532. https://doi.org/10.1016/j.arabjc.2020.08.026
2019
Rodríguez-Solana R., Coelho N., Santos-Rufo A., Gonçalves S., Pérez-Santín E. & Romano A., 2019. The Influence of in vitro gastrointestinal digestion on the chemical composition and antioxidant and enzyme inhibitory capacities of carob liqueurs obtained with different elaboration techniques. Antioxidants, 8(11), 563. https://doi.org/10.3390/antiox8110563
Costa; Medronho; Filipe; Mira; Lindman; Edlund & Norgren., 2019. Emulsion Formation and Stabilization by Biomolecules: The Leading Role of Cellulose. Polymers (Basel), 11, 1570. https://www.mdpi.com/2073-4360/11/10/1570
Singh, Poonam; Magalhães, Solange; Alves, Luis; Antunes, Filipe; Miguel, Maria; Lindman, Björn & Medronho, Bruno., 2019. Cellulose-based edible films for probiotic entrapment. Food Hydrocoll, 88, 68–74. https://doi.org/10.1016/j.foodhyd.2018.08.057
Magalhães S., Alves L., Medronho B., Romano A., Coelho J.F.J. & Norgren M., 2019. Brief overview on bio-based adhesives and sealants. Polymers, 11: 1685 (Artigo de revisão). https://doi.org/10.3390/polym11101685
Medronho B., Filipe A., Napso S., Rein D.M., Pereira R.F.P., de Zea Bermudez V., Romano A. & Cohen Y., 2019. Silk fibroin dissolution in tetrabutylammonium hydroxide aqueous solution. Biomacromolecules, 20: 4107-4116. https://doi.org/10.1021/acs.biomac.9b00946
Gonçalves S., Mansinhos I., Rodríguez-Solana R., Pérez-Santín E., Coelho N. & Romano A., 2019. Elicitation improves rosmarinic acid content and antioxidant activity in Thymus lotocephalus shoot cultures. Industrial Crops and Products, 137: 214-220. https://doi.org/10.1016/j.indcrop.2019.04.071
Gonçalves S., Moreira E., Andrade P.B, Valentão P. & Romano A., 2019. Effect of in vitro gastrointestinal digestion on the total phenolic contents and antioxidant activity of wild Mediterranean edible plants extracts. European Food Research and Technology, 245: 753-762. https://doi.org/10.1007/s00217-018-3197-y
Rodríguez-Solana R., Salgado J.M., Pérez-Santín E. & Romano A., 2019. Effect of carob variety and roasting on the antioxidant capacity, and the phenolic and furanic contents of carob liquors. Journal of the Science of Food and Agriculture, 99: 2697–2707. https://doi.org/10.1002/jsfa.9437
Aliaño-González, M.J.; Ferreiro-González, M.; Barbero, G.F.; Palma, M., 2019. Novel Method Based on Ion Mobility Spectrometry Sum Spectrum for the Characterization of Ignitable Liquids in Fire Debris. Talanta, 199, 189–194. https://doi.org/10.1016/j.talanta.2019.02.063
Aliaño-González, M.J.; Ferreiro-González, M.; Espada-Bellido, E.; Palma, M.; Barbero, G.F., 2019. Screening Method Based on Headspace-Ion Mobility Spectrometry to Identify Adulterated Honey. Sensors, 19, 1621. https://doi.org/10.3390/s19071621
Aliaño-González, M.J.; Ferreiro-González, M.; Espada-Bellido, E.; Palma, M.; Barbero, G.F., 2019. A Screening Method Based on Visible-NIR Spectroscopy for the Identification and Quantification of Different Adulterants in High-Quality Honey. Talanta, 203, 235–241. https://doi.org/10.1016/j.talanta.(2019).05.067
Ferreiro-González, M.; Amores-Arrocha, A.; Espada-Bellido, E.; Aliaño-Gonzalez, M.J.; Vázquez-Espinosa, M.; González-de-Peredo, A.V.; Sancho-Galán, P.; Álvarez-Saura, J.Á.; Barbero, G.F.; Cejudo-Bastante, C., 2019. Escape ClassRoom: Can You Solve a Crime Using the Analytical Process? Journal Chemical Education, 96, 267–273. https://doi.org/10.1021/acs.jchemed.8b00601
Martins, I.M., Meirinho, S., Costa, R., Cravador, A., Choupina, A., 2019. Cloning, characterization, in vitro and in planta expression of a necrosis-inducing Phytophthora protein 1 gene npp1 from Phytophthora cinnamomi. Molecular Biology Reports, 46(6), 6453–6462. https://doi.org/10.1007/s11033-019-05091-0
Nunes, F.D., Sousa, F.M.G., Leitão, J.M.N., 2019. Characterization and performance analysis of generalized BOC modulations for GNSS. Navigation, Journal of the Institute of Navigation, 66(1), 185–197. http://dx.doi.org/10.1002/navi.279
Fazenda, P., Pereira, R., Fonseca, M., Carlier, J., Leitão, J., 2019. Identification and validation of microsatellite markers in strawberry tree (Arbutus unedo L.). Turkish Journal of Agriculture and Forestry, 43(4), 430–436. https://doi.org/10.3906/tar-1807-164
2018
Jung, Thomas; Duran, Alvaro; Sanfuentes, Eugenio; Schena, Leonardo; Mosca, Saveria; Fajardo, Sebastian; González, Mariela; Navarro Ortega, Angella; Bakonyi, Jozsef; Seress, Diana; Tomšovský, Michal; Cravador, Alfredo; Maia, Cristiana; Horta Jung, Marília. Diversity of Phytophthora species in Valdivian rainforests and association with severe dieback symptoms. Forest Pathology, 48(5) (2018). DOI: 10.1111/efp.12443
Alves, Luis; Medronho, Bruno; Filipe, Alexandra; E. Antunes, Filipe; Lindman, Björn; Topgaard, Daniel; Davidovich, Irina & Talmon, Yeshayahu., 2018. New Insights on the Role of Urea on the Dissolution and Thermally-Induced Gelation of Cellulose in Aqueous Alkali. Gels, 4, 87. http://www.mdpi.com/2310-2861/4/4/87
Singh, Poonam; Medronho, Bruno; Santos, Tiago dos; Nunes-Correia, Isabel; Granja, Pedro; Miguel, Maria G. & Lindman, Björn., 2018. On the viability, cytotoxicity and stability of probiotic bacteria entrapped in cellulose-based particles. Food Hydrocoll, 82, 457–465. https://doi.org/10.1016/j.foodhyd.2018.04.027
Melro, Elodie; Alves, Luis; Antunes, Filipe E. & Medronho, Bruno., 2018. A brief overview on lignin dissolution. J. Mol. Liq, 265, 578–584. https://doi.org/10.1016/j.molliq.2018.06.021
Singh, Poonam; Medronho, Bruno; Valente, Artur J. M.; Miguel, Maria G. & Lindman, Björn., 2018. Exploring the prebiotic effect of cyclodextrins on probiotic bacteria entrapped in carboxymetyl cellulose-chitosan particles. Colloids Surfaces B Biointerfaces, 168, 156–162. http://dx.doi.org/10.1016/j.colsurfb.2017.12.014
Singh, Poonam; Medronho, Bruno; Miguel, Maria G. & Esquena, Jordi., 2018. On the encapsulation and viability of probiotic bacteria in edible carboxymethyl cellulose-gelatin water-in-water emulsions. Food Hydrocoll, 75, 41–50. https://doi.org/10.1016/j.foodhyd.2017.09.014
Haffner, F. B.; Canilho, N.; Medronho, B.; Gardiennet, C.; Gansmüller, A. & Pasc, A., 2018. In situ follow-up of hybrid alginate–silicate microbeads formation by linear rheology. Phys. Chem. Chem. Phys, 20, 11819–11825. https://doi.org/10.1039/C8CP00073E
Medronho B., Filipe A., Costa C., Romano A., Lindman B., Edlund H. & Norgren M., 2018. Microrheology of novel cellulose stabilized oil-in-water emulsions. Journal of Colloid and Interface Science, 531: 225-232. https://doi.org/10.1016/j.jcis.2018.07.043
Rodríguez-Solana R., Galego L.R., Pérez-Santín E. & Romano A., 2018. Production method and varietal source influence the volatile profiles of spirits prepared from fig fruits (Ficus carica L.). European Food Research and Technology, 244: 2213-2229. https://doi.org/10.1007/s00217-018-3131-3
Aghraz A., Gonçalves S., Rodríguez-Solana R., Ait Dra L., Di Stefano V., Dugo G., Cicero N., Larhsini M., Markouk M. & Romano A., 2018. Antioxidant activity and enzymes inhibitory properties of several extracts from two Moroccan Asteraceae species. South African Journal of Botany, 118: 58-64. https://doi.org/10.1016/j.sajb.2018.06.017
Jara-Palacios M.J., Gonçalves S., Hernanz D., Heredia F.J. & Romano A., 2018. Effects of in vitro gastrointestinal digestion on phenolic compounds and antioxidant activity of different white winemaking byproducts extracts. Food Research International, 109: 433-439. https://doi.org/10.1016/j.foodres.2018.04.060
Pereira A., Duarte H., Nosrati P., Gubitosi M., Gentile L., Romano A., Medronho B. & Olsson U., 2018. Cellulose gelation in NaOH solutions is due to cellulose crystallization. Cellulose, 25: 3205–3210. https://doi.org/10.1007/s10570-018-1794-3
Gonçalves S., Medronho J., Moreira E., Grosso C., Andrade P.B., Valentão P. & Romano A., 2018. Bioactive properties of Chamaerops humilis L. - antioxidant and enzyme inhibiting activities of extracts from leaves, seeds, pulp and peel. 3 Biotech, 8: 88-96. https://doi.org/10.1007/s13205-018-1110-9
Rodríguez-Solana R., Carlier J.D., Costa M.C. & Romano A., 2018. Multi-element characterization of carob, fig and almond liqueurs by MP-AES. Journal of the Institute of Brewing, 124: 300–309. https://doi.org/10.1002/jib.495
Aliaño-González, M.J.; Ferreiro-González, M.; Barbero, G.F.; Ayuso, J.; Palma, M.; Barroso, C.G., 2018. Study of the Weathering Process of Gasoline by ENose. Sensors, 18, 139. https://doi.org/10.3390/s18010139
Aliaño-González, M.J.; Ferreiro-González, M.; Barbero, G.F.; Ayuso, J.; Álvarez, J.A.; Palma, M.; Barroso, C.G., 2018. An Electronic Nose Based Method for the Discrimination of Weathered Petroleum-Derived Products. Sensors, 18, 2180. https://doi.org/10.3390/s18072180
Aliaño-González, M.J.; Ferreiro-González, M.; Barbero, G.F.; Palma, M.; Barroso, C.G., 2018. Application of Headspace Gas Chromatography-Ion Mobility Spectrometry for the Determination of Ignitable Liquids from Fire Debris. Separations, 5, 41. https://doi.org/10.3390/separations5030041
Ferreiro-González, M.; Aliaño-González, M.J.; Barbero, G.F.; Palma, M.; Barroso, C.G., 2018. Characterization of Petroleum-Based Products in Water Samples by HS-MS. Fuel, 222, 506–512. https://do.org/10.1016/j.fuel.2018.02.127
Jung, T., Durán, A., Sanfuentes von Stowasser, E., .Maia, C., Horta Jung, M., 2018. Diversity of Phytophthora species in Valdivian rainforests and association with severe dieback symptoms. Forest Pathology, 48(5), 12443. https://doi.org/10.1111/efp.12443